Premium
Verification of the interaction between peptide T and CD4 using surface plasmon resonance
Author(s) -
Ramsdale Tracie E.,
Andrews Peter R.,
Nice Edouard C.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80657-g
Subject(s) - surface plasmon resonance , peptide , monoclonal antibody , chemistry , infectivity , receptor–ligand kinetics , biophysics , in vitro , receptor , biochemistry , virology , biology , antibody , virus , immunology , nanoparticle , materials science , nanotechnology
Peptide T is currently in phase II clinical trials for the treatment of AIDS‐associated dementia. Its putative mode of action is inhibition of binding of the HIV envelope protein (gp120) to its cellular receptor (CD4), thus preventing viral infectivity and gp120‐induced neuronal toxicity. However, a number of reports have appeared in the literature which have failed to observe any inhibitory activity of Peptide T on CD4‐gp120 binding, thus casting doubt on this hypothesis. This study uses a novel biosensor technique to demonstrate that Peptide T does bind to CD4 and that this binding can be specifically inhibited by an anti‐CD4 monoclonal antibody. A detailed analysis of the kinetics of the interaction is presented.