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A comparative evaluation of the metabolic profiles of normal and inflammatory knee‐joint synovial fluids by high resolution proton NMR spectroscopy
Author(s) -
Naughton Declan P.,
Haywood Rachel,
Blake David R.,
Edmonds Sally,
Hawkes Geoffrey E.,
Grootveld Martin
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80636-9
Subject(s) - synovial fluid , knee joint , chemistry , nuclear magnetic resonance spectroscopy , spectroscopy , medicine , pathology , endocrinology , osteoarthritis , surgery , physics , stereochemistry , quantum mechanics , alternative medicine
High resolution 1 H NMR spectroscopy has been employed to investigate the metabolic profile of heatlhy human knee‐joint synovial fluid (SF) and the biochemical data acquired have been compared with those of matched serum, and inflammatory knee‐joint SF samples. Results obtained indicate that the healthy human knee‐joint has a hypoxic status (high lactate level when expressed relative to that of paired serum) that is milder than that of the inflamed human knee‐joint. Moreover, normal SF differs from that of inflammatory SF in that it contains little or no NMR‐detectable lipoprotein‐associated fatty acids and ‘acute‐phase’ glycoproteins, an observation reflecting the limited passage of these macromolecules from plasma into the synovial space in healthy subjects.