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Two structural genes are encoding malate synthase isoenzymes in Saccharomyces cerevisiae
Author(s) -
Fernandez Ernestina,
Fernandez Maria,
Rodicio Rosaura
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80601-p
Subject(s) - malate synthase , saccharomyces cerevisiae , glyoxylate cycle , gene , biology , biochemistry , malate dehydrogenase , mutant , yeast , atp synthase , enzyme , isozyme , microbiology and biotechnology , allantoin , isocitrate lyase
We report on the isolation of a gene encoding yeast malate synthase. A yeast genomic library was screened using a probe homologous to the yeast enzyme obtained by the polymerase chain reaction. The nucleotide sequence of the cloned gene was determined. Computer analysis showed that the isolated gene is identical to the one previously described as DAL7 , which is involved in allantoin metabolism [Mol. Cell. Biol. 9 (1989) 3231‐3243]. Enzymatic activities of multicopy transformants, Southern analysis and disruption mutants predict the existence of two genes encoding malate synthases that are differentially regulated at the transcriptional level. Malate synthase; Glyoxylate pathway; Allantoin metabolism; Gene disruption.