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Isolation of two members of the rat MAP kinase kinase gene family
Author(s) -
Otsu Masayuki,
Terada Yasuhiko,
Okayama Hiroto
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80596-m
Subject(s) - map3k7 , map2k7 , cyclin dependent kinase 4 , kinase , gene , map kinase kinase kinase , mitogen activated protein kinase kinase , cyclin dependent kinase 9 , ask1 , cyclin dependent kinase 2 , biology , genetics , microbiology and biotechnology , protein kinase a , chemistry
Mitogen‐activated protein (MAP) kinase kinase (MAPKK) is a recently characterized activator of MAP kinase (MAPK), and is considered to be regulated by a protooncogene product c‐Raf‐1. It is, however, unclear whether the signals originating from c‐Raf‐1 utilize this phosphorylation cascade to lead to oncogenesis. To clarify this point, we isolated rat MAPKK cDNAs, and identified two distinct cDNAs encoding MAPKK and a highly related kinase, both with molecular weights of 4̃5 kDa (MEK1 and MEK2). Genomic Southern blot analyses suggested that MAPKK. may form a large gene family.