Premium
Synechocystis 6803 plastocyanin isolated from both the cyanobacterium and E. coli transformed cells are identical
Author(s) -
Hervás Manuel,
Navarro Francisco,
Navarro José A.,
Chávez Sebastián,
Díaz Antonio,
Florencio Francisco J.,
De la Rosa Miguel A.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80558-c
Subject(s) - plastocyanin , synechocystis , chemistry , cyanobacteria , escherichia coli , biochemistry , bacteria , biology , photosystem i , photosynthesis , genetics , gene , photosystem ii
Native plastocyanin from Synechocystis 6803 has been isolated and purified to electrophoretic homogeneity. The corresponding gene ( petE ) has been cloned and expressed in E. coli , thus leading to a protein completely identical to plastocyanin purified from the cyanobacterial cells. The petE gene product is correctly processed in E. coli as deduced from the N‐terminal amino acid sequences. These results, along with the identical physicochemical and kinetic properties of the two protein preparations, confirm that expression of petE in E. coli is an adequate tool to address the study of Synechocystis plastocyanin by site‐directed mutagenesis.