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Verapamil and diltiazem inhibit receptor‐operated calcium channels and intracellular calcium oscillations in rat hepatocytes
Author(s) -
Striggow Frank,
Bohnensack Ralf
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80542-3
Subject(s) - diltiazem , verapamil , nifedipine , phenylephrine , calcium channel , t type calcium channel , calcium , extracellular , chemistry , voltage dependent calcium channel , medicine , calcium in biology , l type calcium channel , endocrinology , intracellular , vasopressin , vasopressin receptor , p type calcium channel , pharmacology , receptor , biology , antagonist , biochemistry , blood pressure
Fura‐2 loaded rat hepatocytes were used to determine whether the L‐type channel blockers, verapamil and diltiazem, affect receptor‐operated calcium channels (ROCCs). The flux through ROCCs was followed by quenching of fura‐2 fluorescence due to the influx of extracellular Mn 2+ induced by vasopressin. Verapamil as well as diltiazem inhibited vasopressin‐stimulated Mn 2+ influx in a dose‐dependent manner up to 60% at concentrations of 200–400 μM. Furthermore, both inhibitors decreased significantly the frequency of phenylephrine‐induced oscillation of [Ca 2+ ] i . The experimental findings indicate that L‐type channel blockers inhibit ROCCs in rat hepatocytes.