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Evidence for a membrane skeleton in higher plants
Author(s) -
Faraday Christopher D.,
Spanswick Roger M.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80536-4
Subject(s) - polyclonal antibodies , spectrin , membrane , chemistry , differential centrifugation , centrifugation , aqueous solution , chromatography , fraction (chemistry) , membrane protein , biochemistry , antibody , biology , organic chemistry , immunology , cell , cytoskeleton
A fraction enriched in plasma membranes was isolated from rice roots by differential centrifugation and aqueous polymer two‐phase partitioning. Analysis of the fraction by SDS‐PAGE showed the presence of several low mobility polypeptides ( M r > 100 kDa). One of these polypeptides ( M r ~ 230 kDa) was specifically recognized by polyclonal antibodies to human erythrocyte spectrin. This finding suggests that a higher plant spectrin‐based membrane skeleton may be preserved and studied using high‐purity plasma membrane fractions obtained by aqueous polymer two‐phase partitioning.