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Characterization of the alternative oxidase protein in the yeast Hansenula anomala
Author(s) -
Sakajo Shigeru,
Minagawa Nobuko,
Yoshimoto Akio
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80535-3
Subject(s) - alternative oxidase , complementary dna , yeast , biochemistry , biology , antimycin a , mitochondrial respiratory chain , microbiology and biotechnology , saccharomyces cerevisiae , enzyme , mitochondrion , gene
The cyanide‐resistant respiratory pathway is induced by respiratory inhibitors in the yeast Hansenula anomala . A monoclonal antibody against the alternative oxidase in the higher plant Sauromatum guttatum cross‐reacted with a 36‐kDa mitochondrial protein induced by antimycin A in H. anomala and with a protein encoded by a cDNA which was previously cloned for an antimycin A‐inducible mRNA in the yeast. There was a similarity in the amino acid sequence between the cDNA‐encoded protein and the plant alternative oxidase protein. We propose that the 36‐kDa mitochondrial protein encoded by the cDNA is a component of alternative oxidase in H. anomala .