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Cyclic ADP‐ribose‐induced Ca 2+ release from rat brain microsomes
Author(s) -
White Alison M.,
Watson Stephen P.,
Galione Antony
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80524-x
Subject(s) - cyclic adp ribose , ryanodine receptor , nad+ kinase , microsome , second messenger system , endogeny , calcium , inositol trisphosphate , chemistry , sea urchin , metabolite , biochemistry , inositol , biophysics , biology , enzyme , microbiology and biotechnology , endoplasmic reticulum , receptor , cd38 , stem cell , organic chemistry , cd34
Cyclic ADP‐ribose (cADPR), an endogenous NAD + metabolite in many mammalian and invertebrate tissues, is a potent mediator of calcium mobilization in sea urchin eggs. Our results show that cADPR also stimulates calcium release from rat brain microsomes, marked release occurring over the concentration range 10–250 nM. This is not inhibited by concentrations of heparin which completely abolish inositol 1,4,5‐trisphosphate (IP 3 )‐induced Ca 2+ release. Ryanodine (100μM) inhibits the cADPR response. Our results are consistent with cADPR being an endogenous messenger mediating Ca 2+ release from ryanodine‐sensitive pools in brain.