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Presence of lipocortins I and IV, but not II and VI, in human platelets
Author(s) -
Eldering Joyce A.,
Kocher Markus,
Clemetson Jeannine M.,
Clemetson Kenneth J.,
Frey Felix J.,
Frey Brigitte M.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80518-y
Subject(s) - platelet , cytosol , chemistry , thrombin , annexin , biochemistry , lysis , annexin a1 , covalent bond , platelet lysate , membrane , enzyme , biology , cell , in vitro , immunology , organic chemistry
The present investigation revealed the presence of lipocortins I and IV, but not lipocortins II and VI, in human platelets. Lipocortin I was found in the Triton‐soluble fraction of both resting and thrombin‐activated platelets and was not covalently bound to skeletal components. Without detergents, when resting platelets were lysed and fractionated in the absence of Ca 2+ , lipocortin I was found only in the cytosolic fraction, whereas, in the presence of Ca 2+ , lipocortin I was associated only with the crude particulate and not with the membrane nor the cytosolic fractions.