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Preparations of Rp ‐cyclic adenosine 3',5'‐phosphorothioate ( Rp ‐cAMPS) can contain biologically active amounts of adenosine
Author(s) -
Musgrave I.F.,
Genieser H.G.,
Maronde E.,
Seifert R.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80517-x
Subject(s) - adenosine , xanthine , chemistry , adenosine receptor , superoxide , adenosine a1 receptor , receptor , biochemistry , agonist , enzyme
Superoxide anion (O 2 .− ) production from human neutrophils stimulated by N ‐formyl‐ l ‐methionyl‐ l ‐leucyl‐ l ‐phenylalanine (fMLP, 1 μM) was inhibited by preparations of the inhibitor of cAMP‐dependent protein kinase, Rp ‐cyclic adenosine 3',5'‐phosphorothioate ( Rp ‐cAMPS, 100 μM). This effect of Rp ‐cAMPS was reversed by xanthine amine congener (0.1 μM), an adenosine receptor antagonist, and by low concentrations of adenosine desaminase (0.02 mg/ml). HPLC analysis shows that these preparations of Rp ‐cAMPS contained concentrations of adenosine which could produce significant inhibition of fMLP‐induced O 2 .− production. These results suggest that Rp ‐cAMPS should be used with caution in cells or tissues containing adenosine receptors, and that preparations of Rp ‐cAMPS should be treated with adenosine desaminase before use to avoid activation of adenosine receptors.