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The 5' untranslated region of the human γ‐glutamyl transferase mRNA contains a tissue‐specific active translational enhancer
Author(s) -
Diederich Marc,
Wellman Maria,
Visvikis Athanase,
Puga Alvaro,
Siest Gérard
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80490-l
Subject(s) - three prime untranslated region , enhancer , messenger rna , microbiology and biotechnology , untranslated region , five prime untranslated region , translational regulation , biology , chemistry , genetics , translation (biology) , gene , gene expression
We report the functional and structural analysis of the 5' untranslated region (5'UTR) of human hepatoma HepG2 γ‐glutamyltransferase (GGT) mRNA. Transient expression of a hybrid GGT‐luciferase gene in HepG2, MIA‐Pa‐Ca‐2 and MG 63 cell lines shows that this 5'UTR acts as a tissue‐specific translational enhancer. Evidence for transcripts with multiple 5'UTR coding for HepG2 GGT was obtained by RNase protection. Computer analysis of this 5'UTR detected the existence of a stable stem and loop structure containing multiple steroid modulatory elements.