Premium
Yeast coatomer contains a subunit homologous to mammalian β'‐COP
Author(s) -
Harter Cordula,
Draken Esther,
Lottspeich Friedrich,
Wieland Felix T.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80487-f
Subject(s) - yeast , protein subunit , homologous chromosome , cytosol , biochemistry , polyacrylamide gel electrophoresis , chemistry , urea , saccharomyces cerevisiae , peptide , microbiology and biotechnology , biology , enzyme , gene
The homologue of the mammalian coatomer complex was isolated from yeast cytosol and separated on a modified urea‐containing SDS‐polyacrylamide gel system. An additional band in the 100 kDa molecular weight range appeared when compared to the protein pattern obtained in conventional Laemmli gels, exactly as observed for mammalian coatomer. Cross‐reactivity with an anti‐peptide antibody raised against the C‐terminus of β‐COP from bovine, and N‐terminal sequence analysis, revealed that this protein from yeast is related to β‐COf from mammals.