Premium
pp59 fyn and pp62 c‐yes are enriched in SH‐SY5Y neuroblastoma growth cones but do not associate to the 38 kDa protein which complexes with pp60 c src and pp60 c‐srcN
Author(s) -
Meyerson Gabrielle,
Påhlman Sven
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80475-a
Subject(s) - fyn , tyrosine kinase , immunoprecipitation , kinase , biology , microbiology and biotechnology , growth cone , cell growth , sh sy5y , neuroblastoma , gene isoform , proto oncogene tyrosine protein kinase src , biochemistry , cell culture , signal transduction , gene , genetics , axon
The tyrosine‐specific kinases pp60 c‐src and pp60 c‐srcN (pp60 src ) are slightly enriched and activated in growth cones isolated from neuronally differentiating SH‐SY5Y neuroblastoma cells. In the growth cones the two src isoforms are associated with a 38 kDa protein. In this report, we have compared the subcellular distribution of pp59 fyn and pp62 c‐yes with that of pp60 src in differentiating SH‐SY5Y cells. Like pp60 src , the other two tyrosine kinases were slightly enriched and activated in the growth cones as compared to the levels in the cell bodies. The kinase activites were 3‐ to 4‐times higher in growth cones than in cell bodies. However, only pp60 src formed a complex with the 38 kDa protein while immunoprecipitation of pp59 fyn brought down an additional protein of 90 kDa. This may suggest that these related tyrosine kinases have different substrates and in part mediate different cellular responses in the growth cones of differentiating SH‐SY5Y cells.