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Enzymes of the cyclic GMP metabolism in bovine retina
Author(s) -
Gaidarov I.O.,
Suslov O.N.,
Abdulaev N.G.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80444-y
Subject(s) - biochemistry , enzyme , complementary dna , peptide sequence , nucleotide , amino acid , biology , yeast , nucleic acid sequence , chemistry , microbiology and biotechnology , gene
Guanylate kinase (EC 2.7.4.8) catalyzing the reaction GMP + ATP = GDP + ADP, was purified to homogeneity from bovine retina. Using oligonucleotides based on the amino acid sequence of this enzyme, the cDNA encoding guanylate kinase (GK) was isolated and its nucleotide sequence was determined. Expression of the GK cDNA in E. coli , and the purification and functional characterization of the expressed enzyme are presented. It is shown that bovine retinal GK, like its yeast counterpart, contains the characteristic glycine‐rich motif and all the amino acids involved in GMP binding. Bovine retinal enzyme is extended for several amino acid residues both at the N‐ and C‐termini, compared to the yeast enzyme.