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Activation of snake venom metalloproteinases by a cysteine switch‐like mechanism
Author(s) -
Grams Frank,
Huber Robert,
Kress Lawrence F.,
Moroder Luis,
Bode Wolfram
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80443-x
Subject(s) - matrix metalloproteinase , venom , mechanism (biology) , cysteine , snake venom , chemistry , metalloproteinase , microbiology and biotechnology , biophysics , biochemistry , biology , enzyme , philosophy , epistemology
The cDNAs of several snake venom zinc endopeptidases code for a putative propeptide, which includes the conserved cysteine‐containing sequence PKMCGVT. It has been suggested that binding of the cysteine thiol function to the active‐site zinc, resulting in inactivation of the catalytic domain, occurs in a mode similar to the ‘cysteine switch’ mechanism proposed for matrix metalloproteinases. In order to confirm this hypothesis, inhibition kinetics have been performed on the metalloproteinase adamalysin II of the venom of the snake Crotalus adamanteus using several cysteine peptides. Among these the synthetic hexapeptide PKMCGV‐NH 2 , corresponding to the conserved sequence portion of the known propeptides, was found to be by far the strongest inhibitor of this proteinase with a K i of 3.4 μM. The inhibitory potencies of an equivalent peptide with the l ‐Cys replaced by a d ‐Cys or by an l ‐Ser as well as of reduced glutathione, cysteine and two unrelated cysteine peptides were by one to two orders of magnitudes lower. These findings strongly support a cysteine switch‐like mechanism even for activation of the snake venom metalloproteinases.