Premium
RPII15 codes for the M r 15,000 subunit 9 of Drosophila melanogaster RNA polymerase II
Author(s) -
Liu Zhihong,
Kontermann Roland E.,
Schulze Rüdiger A.,
Petersen Gabriele,
Bautz Ekkehard K.F.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80442-w
Subject(s) - polymerase , microbiology and biotechnology , biology , drosophila melanogaster , specificity factor , gene , protein subunit , polymerase chain reaction , rna polymerase , rna polymerase i , sequence analysis , coding region , genomic dna , peptide sequence , inverse polymerase chain reaction , genetics , nested polymerase chain reaction , rna , rna dependent rna polymerase
The RPII15 gene product of Drosophila melanogaster , which has recently been identified by sequence comparison, possesses a high similarity to subunit 9 of yeast RNA polymerase II. Using the polymerase chain reaction the coding region of RPII15 was isolated from genomic DNA of adult flies. Sequence analysis shows four amino acid substitutions in comparison to the previously reported sequence. Antisera were generated against bacterially expressed RPII15 and were used for immunoblotting experiments with RNA polymerase II of Drosophila melanogaster . This analysis identified the M r , 15,000 subunit 9 as gene product of RPII15 .