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Regulation of CCK mRNA in the human neuroepithelioma cell line SK‐N‐MCIXC in response to second messenger activators
Author(s) -
Mania-Farnell Barbara L.,
Merrill Bradley J.,
Konings Pierre N.M.,
Davis Thomas P.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80440-6
Subject(s) - protein kinase c , second messenger system , messenger rna , northern blot , cholecystokinin , microbiology and biotechnology , protein kinase a , cell culture , phosphodiesterase , phorbol , complementary dna , kinase , gene expression , signal transduction , chemistry , biology , biochemistry , receptor , enzyme , gene , genetics
Regulation of cholecystokinin (CCK) expression was studied in the human neuroepithelioma cell line SK‐N‐MCIXC. The cells were treated with the phosphodiesterase inhibitor isobutyl‐methylxanthine and the tumor promoting phorbol ester, phorbol‐12‐myristate 13‐acetate; activators of the cyclic AMP (cAMP) and protein kinase C (PKC) second messenger pathways, respectively. Levels of CCK mRNA were determined after 6, 12 and 24 hour drug treatments, with Northern blot analysis using human CCK cDNA hybridization probes. Activation of both cAMP and PKC second messenger pathways increased CCK mRNA levels in SK‐N‐MCIXC cells. These results indicate that the levels of CCK mRNA in SK‐N‐MCIXC cells are regulated by cAMP and PKC dependent mechanisms.