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Immediate‐early gene induction by repetitive mechanical but not electrical activity in adult rat cardiomyocytes
Author(s) -
Kubisch Christian,
Wollnik Bernd,
Maass Alexander,
Meyer Rainer,
Vetter Hans,
Neyses Ludwig
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80434-v
Subject(s) - contraction (grammar) , stimulation , immediate early gene , muscle hypertrophy , medicine , endocrinology , c fos , chemistry , muscle contraction , gene , microbiology and biotechnology , biology , gene expression , biochemistry
Mechanical factors are thought to play an important role in the induction of myocardial hypertrophy. Yet, it is not known whether active contraction induces genes that probably represent initial steps in the hypertrophic response in the adult myocardium ‐ and if so, whether the mechanical or the electrical component of the twitch governs this response. We therefore investigated whether electrical stimulation of contraction was able to induce the immediate‐early genes (IEGs) egr‐1 and c‐fos in adult rat cardiomyocytes. Cyclical contraction led to an increase in egr‐1 and c‐fos mRNA levels within 30 min. Full inhibition of contraction during electrostimulation by the Ca 2+ ‐desensitizer 2,3‐butanedione monoxime (BDM) totally blocked this IEG‐response without altering membrane potential. These data suggest that in adult myocardium, the mechanical rather than the electrical activity is responsible for the IEG‐response during active twitch.