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The genomic region encoding toxin gamma from the scorpion Tityus serrulatus contains an intron
Author(s) -
Becerril B.,
Corona M.,
Mejía M.C.,
Martin B.M.,
Lucas S.,
Bolivar F.,
Possani L.D.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80428-w
Subject(s) - scorpion , scorpion toxin , intron , biology , genetics , toxin , computational biology , gene , venom , ecology
The gene encoding toxin gamma from the scorpion, Tityus serrulatus , was amplified by PCR from genomic DNA employing synthetic oligonucleotides designed from the reported cDNA sequence. The nucleotide sequence of this gene reveals the presence of an intron of 475 base pairs (bp) which interrupts the region that encodes the signal peptide of the precursor toxin. A comparison of the intron boundary sequences of the gamma toxin gene with ones from other arachnid genes is also presented.