Premium
Cloning and disruption of the gene encoding yeast mitochondrial chaperonin 10, the homolog of E. coli groES
Author(s) -
Rospert Sabine,
Junne Tina,
Glick Benjamin S.,
Schatz Gottfried
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80419-u
Subject(s) - chaperonin , groes , cloning (programming) , yeast , gene , mitochondrial dna , chemistry , escherichia coli , groel , microbiology and biotechnology , genetics , biology , computer science , programming language
The mitochondrial chaperonin system consists of chaperonin 60 (also termed hsp60), which is homologous to E. coli groEL, and chaperonin 10, which is homologous to E. coli groES. In yeast, chaperonin 60 function has been shown to be essential for viability. We report here that the same is true for chaperonin 10. We have cloned, sequenced and disrupted the nuclear chaperonin 10 gene CPN10 from Saccharomyces cerevisiae . This gene encodes a protein of 11,372 Da that is imported into the mitochondrial matrix without detectable cleavage. Haploid cells lacking a functional copy of CPN10 fail to grow at temperatures between 23 and 37° C.