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Catabolite inactivation of the yeast maltose transporter is due to proteolysis
Author(s) -
Lucero Pilar,
Herweijer Marga,
Lagunas Rosario
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80397-d
Subject(s) - maltose , transporter , biochemistry , catabolism , catabolite repression , yeast , saccharomyces cerevisiae , glucose transporter , proteolysis , chemistry , maltose binding protein , biology , recombinant dna , sucrose , mutant , metabolism , enzyme , gene , fusion protein , insulin , endocrinology
The maltose transport capacity of fermenting Saccharomyces cerevisiae rapidly decreases when protein synthesis is impaired. Using polyclonal antibodies against a recombinant maltose transporter‐protein we measured the cellular content of the transporter along this inactivation process. Loss of transport capacity was paralleled by a decrease of cross‐reacting material which suggests degradation of the transporter. We also show that in ammonium‐starved cells the half‐life of the maltose transporter is 1.3 h during catabolism of glucose and > 15 h during catabolism of ethanol.