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Antibodies against the major brain isofbrms of 14‐3‐3 protein
Author(s) -
Martin Harry,
Patel Yasmina,
Jones David,
Howell Steve,
Robinson Karen,
Aitken Alastair
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80356-y
Subject(s) - gene isoform , acetylation , antiserum , recombinant dna , antibody , biochemistry , posttranslational modification , peptide , biology , chemistry , microbiology and biotechnology , enzyme , gene , immunology
14‐3‐3 proteins are apparently ubiquitous eukaryotic proteins that comprise a large number of isoforms. They have been implicated in the regulation of a wide range of biological processes [reviewed in Aitken et al. (1992) Trends Biochem. Sci. 17,498‐501]. We have raised specific antibodies against each mammalian brain isoform of 14‐3‐3 employing peptides synthesised from the amino‐tenninal regions. The peptides were, like the proteins from mammalian brain, N‐acetylated. The antiserum specific for the epsilon isoform did not recognise the recombinant form of this protein (lacking the N‐acetyl co‐translational modification) expressed in E. coli until it was chemically acetylated.