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Determination of distances involving exchangeable protons from NOESY spectra of two DNA dodecamers
Author(s) -
Michalczyk Ryszard,
Russu Irina M.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80340-z
Subject(s) - two dimensional nuclear magnetic resonance spectroscopy , chemistry , nuclear overhauser effect , proton , mixing (physics) , dna , aqueous solution , spectral line , relaxation (psychology) , crystallography , proton nmr , nuclear magnetic resonance spectroscopy , analytical chemistry (journal) , stereochemistry , physics , chromatography , psychology , social psychology , biochemistry , quantum mechanics , astronomy
Two‐dimensional proton nuclear Overhauser effect (NOESY) spectra were obtained as a function of mixing time for two DNA dodecamers, 5'‐CGCGAATTCGCG‐3' and 5'‐CGCAAATTTGCG‐3', in aqueous solutions. The time evolution of cross‐peak volumes was quantitatively analyzed based on the approximate solution of the relaxation/exchange equation over a range of mixing times from 30 to 150 ms. Inter‐proton distances, involving exchangeable protons in key locations of the structures, were calculated and compared to the distances predicted by the crystal structures. These NMR‐derived distances enhance the number of constraints, and their accuracy, for determination of solution structures of the two DNA dodecamers.