Premium
ras oncogene‐induced transformation of a rat seminal vesicle epithelial cell line produces a marked increase of adenylate cyclase and protein kinase C activities
Author(s) -
Spina A.M.,
Chiosi E.,
Naviglio S.,
Valente F.,
Marchese M.,
Furgi A.,
Metafora S.,
Illiano G.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80315-l
Subject(s) - adenylate kinase , forskolin , cyclase , protein kinase a , biology , enzyme , microbiology and biotechnology , cell culture , effector , protein kinase c , gs alpha subunit , receptor , adenylyl cyclase , kinase , chemistry , signal transduction , biochemistry , genetics
Cells transformed by Kirsten murine sarcoma virus (Ki‐MSV) have basal adenylate cyclase activity (AC) higher than control cells and comparable level of forskolin‐stimulated AC activity. Moreover, a higher protein kinase C (PKC) activity was found to be present in the transformed cells. The molecular mechanism underlying the increase of AC activity was investigated. Our findings strongly suggest that this biochemical event is due to a marked decrease of the α i negative control of the enzyme, even though the α i of transformed cells appears to possess fully functional domains interacting with both the effector enzyme and the agonist‐activated receptor.