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The pH‐dependent anion‐conducting channel of the mitochondrial inner membrane is potently inhibited by zinc ions
Author(s) -
Selwyn Michael J.,
Ng Lay Tin,
Choo Hui Lim
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80311-h
Subject(s) - chemistry , zinc , inner mitochondrial membrane , membrane , phosphate , ion , biophysics , membrane potential , inorganic chemistry , nuclear chemistry , biochemistry , biology , organic chemistry
Zinc is a potent reversible inhibitor of the pH‐dependent anion‐conducting channel in the mitochondrial inner membrane, 50% inhibition was produced by 1.5 μM added Zn 2+ at which point free Zn 2+ was ⩽ 10 −8 M. Inhibition by Zn 2+ is rapid but can be prevented or rapidly reversed by excess EDTA. Concentrations of Zn 2+ higher than 4 μM caused reversal of inhibition to a variable extent depending on the anion. Under these conditions Zn 2+ did not inhibit ribose entry, the phosphate transporter, or the pH‐insensitive component of the NO 3 − uniport.