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Cloning and sequence of the cDNA coding for rat type II Fcγ receptor of mast cells
Author(s) -
Boček Petr,
Pecht Israel
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80302-b
Subject(s) - complementary dna , biology , microbiology and biotechnology , peptide sequence , receptor , gene isoform , coding region , alternative splicing , mast cell , genetics , gene , immunology
A clone encoding the rat type II Fcγ receptor was isolated from a cDNA library of the rat mucosal mast cell line RBL‐2H3 and its sequence determined. The predicted amino acid sequence is highly homologous with the mouse type II as well as rat type III Fcγ receptors. The site of alternative splicing which generates the mouse FcγRb2 isoform is completely conserved. Hence we consider the new sequence to encode a rat FcγRII b2 isoform. In further analogy to the mouse receptor, no consensus motif known to be involved in accessory chain association was observed in the transmembranal domain. The importance of the identification of this receptor for investigation of the immunological stimulation of mast cells is discussed.