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Physical map of the extremely thermophilic bacterium Thermus thermophilus HB27 chromosome
Author(s) -
Tabata Kazuyuki,
Kosuge Takehide,
Nakahara Tadaatsu,
Hoshino Takayuki
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80301-a
Subject(s) - thermus thermophilus , thermophile , thermus , bacteria , biology , genetics , microbiology and biotechnology , chemistry , escherichia coli , gene
The physical map of the chromosome of Thermus thermophilus HB27 was constructed using three restriction enzymes; Eco RI, Ssp I and Mun I by applying pulsed‐field gel electrophoresis techniques. Although the genome size of 1.82 Mb was almost the same as that (1.74 Mb) reported for T. thermophilus HB8 [Borges, K.M., and P.L. Bergquist. (1993) J. Bacteriol. 175, 103‐110], the Mun I cleavage maps were different. A 240 kb plasmid was detected in HB27, and its physical map was also constructed. In addition, several genes were located on the chromosomal physical map.