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A complex of rab3A, SNAP‐25, VAMP/synaptobrevin‐2 and syntaxins in brain presynaptic terminals
Author(s) -
Horikawa Hiroshi P.M.,
Saisu Hideo,
Ishizuka Toru,
Sekine Yoko,
Tsugita Akira,
Odani Shoji,
Abe Teruo
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80281-x
Subject(s) - synaptobrevin , digitonin , syntaxin , exocytosis , chemistry , biochemistry , syntaxin 3 , synaptic vesicle , biology , membrane , vesicle
Two monoclonal antibodies (SPM‐1 and SPM‐2) immunoprecipitate brain N‐type calcium channels. On immunoaffinity chromatography of digitonin extracts of bovine brain membranes on SPM‐1‐ and SPM‐2‐Sepharose, proteins of 36 (syntaxins A and B), 28 and 19 kDa are specifically retained by both columns. Here we show that the 19 and 28 kDa bands contain VAMP/synaptobrevin‐2, and rab3A/smg25A and SNAP‐25, respectively. Since SPM‐1 and SPM‐2 recognize only syntaxins and the 28 kDa band (rab3A/smg25A and SNAP‐25), respectively, the results indicate that all these proteins form a complex. Our results suggest tight linkage between the components involved in neurotransmitter release.