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Lithium γ‐linolenate‐induced cytotoxicity against cells chronically infected with HIV‐1
Author(s) -
Kinchington Derek,
Randall Sharon,
Winther Michael,
Horrobin David
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80277-2
Subject(s) - lipid peroxidation , incubation , thiobarbituric acid , linolenate , cytotoxicity , intracellular , chemistry , antioxidant , vitamin e , lithium (medication) , biochemistry , in vitro , microbiology and biotechnology , biology , endocrinology , fatty acid
Lithium γ‐linolenate (Li‐GLA), was evaluated for its activity in selectively killing H9 cells chronically infected with HIV‐1 RF . After 4 days incubation with Li‐GLA approximately 90% of the H9 RF cells were non‐viable compared to 20% of uninfected H9 cells. The efficacy of the Li‐GLA, in preferentially killing HIV infected cells also correlates with lipid peroxidation, as measured by the intracellular thiobarbituric acid‐reactive material content. The addition of an antioxidant (vitamin E) to the culture medium reduced the toxicity of Li‐GLA. These data indicate that this selective killing effect of cells chronically infected with HIV may be due to the enhanced extent of lipid peroxidation of the added Li‐GLA.