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Interaction of GTP‐binding protein Gq with photoactivated rhodopsin in the photoreceptor membranes of crayfish
Author(s) -
Terakita Akihisa,
Hariyama Takahiko,
Tsukahara Yasuo,
Katsukura Yuki,
Tashiro Hideo
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80272-v
Subject(s) - rhodopsin , gtp' , immunoprecipitation , crayfish , g protein , biophysics , binding protein , gq alpha subunit , protein subunit , microbiology and biotechnology , chemistry , membrane , biology , biochemistry , retinal , receptor , enzyme , gene , fishery
Interaction of G‐protein with photoactivated rhodopsin (Rh ∗ ) in crayfish photoreceptor membranes was investigated by immunoprecipitation using an antibody against rhodopsin. Two kinds of protein were co‐precipitated with rhodopsin. One is an α subunit of class‐q G‐protein (42 kDa, CGqα) which showed light‐induced, dose‐dependent binding to rhodopsin, and the other is an actin‐like protein (44 kDa) with light‐independent binding. Most of the CGqα was available for binding to Rh ∗ but was dissociated from Rh ∗ in the presence of GTPγS. These findings demonstrate that, in the crayfish photoreceptor, a Gq class of G‐protein is activated by Rh ∗ .