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The conformation of tRNA genes
Author(s) -
McLaren A.,
Gonos E.S.,
Carr T.,
Goddard J.P.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80268-y
Subject(s) - deamination , gene , transfer rna , cytosine , biology , chemistry , biochemistry , genetics , rna , enzyme
It has been suggested that eukaryotic tRNA genes might adopt a higher order stem and loop structure to facilitate transcription by interaction of their variably spaced intragenic promoter blocks. Using sodium bisulphite, which reacts specifically with cytosine residues in single‐stranded nucleic acids, no deamination of C in the TTCGAA sequence of the 3' ICR of a tRNA Leu gene could be detected under conditions which caused 60% deamination of cytosine residues within the loop region of a synthetic cruciform cloned in the same negatively supercoiled plasmid vector. We conclude that, under these conditions, such structures occur in tRNA genes very rarely, if at all.