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Cloning and expression of a novel human profilin variant, profilin II
Author(s) -
Honoré Bent,
Madsen Peder,
Andersen Annette H.,
Leffers Henrik
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80262-s
Subject(s) - profilin , complementary dna , microbiology and biotechnology , biology , cloning (programming) , northern blot , skeletal muscle , placenta , gene , biochemistry , genetics , anatomy , fetus , cell , actin cytoskeleton , pregnancy , computer science , programming language , cytoskeleton
We have isolated a 1.7 kbp cDNA encoding a 140 amino acid protein (15.1 kDa, pI 5.91) with a high sequence similarity (62%) to human profilin (profilin I). We have termed this variant profilin II. Northern blot analysis showed that profilin II is highly expressed in brain, skeletal muscle and kidney and less strongly in heart, placenta, lung and liver. In addition, three different transcript lengths were detected. Only one transcript of profilin I was found. The expression level of this was low in brain and skeletal muscle, medium in heart and high in placenta, lung, liver and kidney.