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Fluorescence quenching at interfaces and the permeation of acrylamide and iodide across phospholipid bilayers
Author(s) -
Moro Fernando,
Goñi Félix M.,
Urbaneja M.Angeles
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80257-u
Subject(s) - chemistry , quenching (fluorescence) , permeation , iodide , bilayer , acrylamide , fluorescence , lipid bilayer , phospholipid , aqueous solution , membrane , photochemistry , vesicle , inorganic chemistry , organic chemistry , polymer , biochemistry , physics , quantum mechanics , copolymer
Studies of fluorescence quenching in membrane proteins are complicated by the fact that the barrier effect of the bilayer towards the quenchers is not known with precision. Our studies show that (a) both acrylamide and iodide can permeate the membrane at comparable rates, (b) when quenchers are added externally to a vesicle suspension, the apparent Stern‐Volmer quenching constants for the same fluorophores are lower in the inner than in the outer aqueous compartments, and (c) at least some non‐polar fluorophores embedded in the bilayer are quenched by iodide, but not by acrylamide.