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Glycogen contains phosphodiester groups that can be introduced by UDPglucose:glycogen glucose 1‐phosphotransferase
Author(s) -
Lomako Joseph,
Lomako Wieslawa M.,
Whelan William J.,
Marchase Richard B.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80234-l
Subject(s) - glycogen , phosphotransferase , phosphodiester bond , biochemistry , chemistry , glycogen synthase , glycogen branching enzyme , uridine diphosphate glucose , glycogenesis , glycosyltransferase , phosphorylation , enzyme , rna , gene
Rabbit‐muscle glycogen contains covalently bound phosphorus, equivalent to 1 phosphate group per 208 glucose residues. This often disputed, minor component was previously thought to represent a phosphomonoester group at C‐6 of a glucose residue. Here we show that more than half the phosphorus is present as a phosphodiester, the remainder being monoester. A novel enzyme activity has been found in muscle that can account for the presence of the phosphodiester in glycogen. This is a UDPglucose:glycogen glucose 1 ‐phosphotransferase that positions glucose 1 ‐phosphate on C‐6 of glucose residues in glycogen, forming a diester. The phosphomonoester groups present may arise by removal of the glucose residue originally transferred as glucose 1‐phosphate.