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Placental alkaline phosphatase activity is inversely related to cell growth rate in HeLaS3 cervical cancer cells
Author(s) -
Telfer Joan F.,
Green Christopher D.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80229-n
Subject(s) - placental alkaline phosphatase , alkaline phosphatase , cell growth , phosphatase , biology , bromodeoxyuridine , microbiology and biotechnology , enzyme , chemistry , endocrinology , medicine , biochemistry
Placental alkaline phosphatase is an inducible enzyme, expressed in HeLaS3 cells, which has been shown to possess protein phosphotyrosine phosphatase activity. Since phosphotyrosine levels are known to increase in actively dividing cells we sought an inverse correlation between PLAP activity and growth rate in HeLaS3 cells. We found that PLAP inducers, Na‐butyrate, dexamethasone, bromodeoxyuridine and dibutyryl cAMP caused a dose‐dependent reduction in growth rate. Mimosine, an agent that blocks the cell cycle in Gl, caused an increase in PLAP activity whilst the mitogen EGF caused a corresponding decrease in PLAP activity. PLAP activity may therefore be related to cell proliferation rate.