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Internalization and degradation of hepatocyte growth factor in hepatocytes with down‐regulation of the receptor/c‐ Met
Author(s) -
Naka Daiji,
Shimomura Takeshi,
Yoshiyama Yoshiko,
Sato Maki,
Sato Mutsumi,
Ishii Takehisa,
Hara Hiroto
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80212-d
Subject(s) - hepatocyte growth factor , internalization , hepatocyte , receptor , c met , microbiology and biotechnology , chemistry , cell , growth factor , cell growth , cell surface receptor , biology , biochemistry , in vitro
Hepatocyte growth factor (HGF) promotes proliferation of cultured hepatocytes by its interaction with cell surface receptors. In this paper, we examined the metabolic fate of HGF using hepatocytes. Kinetic analysis using [ 125 I]HGF showed that 40% of surface‐bound HGF was rapidly internalized in hepatocytes within 30 min at 37°C. Under these conditions, the amount of HGF‐bound c‐ Met , the high‐affinity receptor, decreased from the cell surface. Furthermore, the internalized HGF was degraded and released from the cells. These results indicate that cell surface‐bound HGF is internalized and degraded by the receptors, including c‐ Met , on hepatocytes.