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Human insulin gene enhancer‐binding proteins in pancreatic α and β cell lines
Author(s) -
Clark Andrew R.,
Petersen Helle V.,
Read Martin L.,
Scott Valerie,
Michelsen Birgitte K.,
Docherty Kevin
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80210-l
Subject(s) - enhancer , human insulin , gene , insulin , chemistry , cell culture , microbiology and biotechnology , biology , biochemistry , gene expression , genetics , endocrinology
Electrophoretic mobility shift assays were performed using oligonucleotides corresponding to known protein binding sites within the human insulin gene enhancer and nuclear extracts from mouse pancreatic α and β cell lines. The results demonstrate that a previously described factor, IUF‐1, binds to three sites at −82 (the CT1 box), −215 (the CT2 box), and −319 (the CT3 box) in the human insulin gene enhancer. IUF‐1 was present only in β but not in α cells, while all other DNA‐binding proteins were present in both cell lines. IUF‐1 may therefore be an important determinant of insulin gene β cell‐specific expression.