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Cell‐specific expression of G q/11 protein and mRNA in rat seminiferous tubules
Author(s) -
Haugen Trine B.,
Paulssen Ruth H.,
Hansson Vidar
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80201-5
Subject(s) - sertoli cell , somatic cell , messenger rna , microbiology and biotechnology , germ cell , gq alpha subunit , biology , phospholipase c , seminiferous tubule , signal transduction , spermatogenesis , g protein , chemistry , gene , endocrinology , biochemistry
As part of a study to elucidate the involvement of G proteins in signal transduction in testicular cells, we have examined the cellular localization of G q/11 within the seminiferous tubules. The somatic cells (Sertoli cells, peritubular cells) contain high amounts of both G q/11 α mRNA and immunoreactive protein. In contrast, very low levels of these G proteins and the corresponding mRNAs are present in the germ cells (pachythene spermatocytes, round spermalids). Thus, in the germ cells, receptor‐regulated inositol phospholipid hydrolysis is not likely to be regulated via G p/11 , but rather through the G o protein, which has been previously shown to be abundant in rat germ cells. Since the somatic cells are nearly devoid of G o , the Gpp(NH)p‐stimulated phospholipase C in these cells is probably regulated by G q and/or g 11 .