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Yeast mitochondrial ATP‐dependent protease: purification and comparison with the homologous rat enzyme and the bacterial ATP‐dependent protease La
Author(s) -
Kutejová Eva,
DurčovÁ Gabriela,
SurovkovÁ Eva,
Kužela Štefan
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80190-6
Subject(s) - protease , biochemistry , saccharomyces cerevisiae , yeast , enzyme , serine protease , mitochondrion , biology , chemistry
Homogenous ATP‐dependent protease has been isolated for the first time from mitochondria of the yeast Saccharomyces cerevisiae . The enzyme molecule consists of six 120 kDa subunits. It is a serine protease with an absolute ATP requirement for its activity. Basic enzymatic characteristics of the yeast protease are similar to those of the corresponding rat mitochondrial enzyme and of the E. coli protease La. The yeast enzyme immunochemically cross‐reacts with the bacterial protease La.