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Inhibition of the degradation of the precursor and of the mature β1 integrin subunit by different protein synthesis inhibitors and by ATP depletion
Author(s) -
Vekeman Sylvie,
Jaspers Martine,
Cassiman Jean-Jacques
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80171-p
Subject(s) - cycloheximide , puromycin , protein subunit , protein degradation , intracellular , protein biosynthesis , microbiology and biotechnology , biochemistry , ubiquitin , proteases , integrin , biology , chemistry , cell , enzyme , gene
A stabilization of both the precursor and the mature β1 integrin subunit was observed in metabolically labeled human skin fibroblasts and Molt‐4 T lymphocytes upon addition of protein synthesis inhibitors or by ATP depletion. Differentia] effects of protein synthesis inhibitors are reported since the slow degradation of the mature β1 subunit was sensitive to cycloheximide but not to puromycin. We also show that the half‐life of the mature subunit was not dependent on intracellular lysosomal degradation or on ubiquitination suggesting that VLA turn‐over occurs at the cell surface and might involve proteins or proteases with short half‐life.