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Detection of a 10 kDa breakdown product containing the C‐terminus of the D 1‐protein in photoinhibited wheat leaves suggests an acceptor side mechanism
Author(s) -
Cánovas Pedro M.,
Barber James
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80147-m
Subject(s) - photoinhibition , cleavage (geology) , n terminus , transmembrane protein , c terminus , chemistry , acceptor , biochemistry , stereochemistry , biology , peptide sequence , amino acid , photosystem ii , gene , paleontology , photosynthesis , receptor , physics , fracture (geology) , condensed matter physics
Photoinhibition of intact leaves of wheat generates a 10 kDa breakdown product which is clearly observed both at 4°C and 25°C. Selective immunoblotting has shown that the 10 kDa fragment contains the C‐terminus of the D 1‐protein and, under the conditions employed, supports an acceptor side mechanism for photoinhibition in vivo. Although a corresponding 23 kDa N‐terminal Dl‐fragment was not detected our results are consistent with the argument that the primary cleavage site is in the loop joining putative transmembrane segments IV and V.