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The catalytic domain of human hepatitis delta virus RNA
Author(s) -
Lee Bao-Shiang,
Wu Huey-Nan,
Huang Tai-Huang
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80138-k
Subject(s) - hepatitis delta , virology , hepatitis a virus , rna , delta , virus , domain (mathematical analysis) , chemistry , biology , hepatitis b virus , biochemistry , physics , gene , mathematics , mathematical analysis , astronomy
We have obtained and analyzed the 600 MHz proton NMR spectra of a 74‐mer RNA derived from the catalytic domain of hepatitis delta virus genomic RNA (HDV RNA) to determine its secondary structure. Deconvolution of the NMR spectrum obtained at 32°C indicates that part of the 74‐mer RNA molecule may exist in multiple conformations in equilibrium. The major conformer contains two A‐U base pairs and 14 ± 2 G‐C base pairs. It appears to contain no standard G‐U base pairs. Our NMR melting study suggests that this conformer has at least two stem‐loop regions. One of the regions has been identified to be a tetra‐loop. We have assigned five imino proton resonances of the tetraloop stem. Our data is consistent with the pseudoknot model of Perrotta and Been.