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Visualization of the morphology of purple membrane surfaces by monoclonal antibody techniques
Author(s) -
Yamaguchi Naoto,
Jinbo Yumiko,
Arai Masao,
Koyama Koichi
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80136-i
Subject(s) - monoclonal antibody , morphology (biology) , visualization , chemistry , antibody , biophysics , computer science , biology , artificial intelligence , immunology , genetics
A monoclonal antibody technique for determining the surface morphology of the purple membrane (PM) has been developed. Two types of antibodies for distinguishing the extracellular surface and the cytoplasmic surface of PM were produced by immunizing the corresponding synthetic peptides to mice. The Langmuir‐Blodgett (LB) method was employed to form a single‐layer two‐dimensional array of PM fragments on an electron microscope grid. The LB film was then treated with the antibodies which had been labeled with colloidal gold particles. Electron microscope observations visualized the morphology of the two surfaces of PM to be rough and smooth regions, respectively; thus the orientation of PM fragments was determined.