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Requirement of the calcium channel β subunit for functional conformation
Author(s) -
Nishimura Seiichiro,
Takeshima Hiroshi,
Hofmann Franz,
Flockerzi Veit,
Imoto Keiji
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80135-h
Subject(s) - r type calcium channel , protein subunit , calcium channel , chinese hamster ovary cell , n type calcium channel , l type calcium channel , interleukin 10 receptor, alpha subunit , voltage dependent calcium channel , calcium , microbiology and biotechnology , complementary dna , chemistry , biology , t type calcium channel , g alpha subunit , biochemistry , receptor , gene , organic chemistry
The cardiac dihydropyridine‐sensitive L‐type calcium channel was stably expressed in Chinese hamster ovary cells by transfecting the rabbit cardiac calcium channel α 1 subunit cDNA with or without coexpression of the β subunit of skeletal muscle calcium channel. Whereas coexpression of the β subunit significantly increased DHP binding activity and calcium channel activity, it did not affect the amount of the α 1 subunit expressed, as judged by RNA blot hybridization analysis and immunoblotting analysis. The results suggest that association with the β subunit is necessary for the α 1 subunit protein to take a proper conformation suitable for a functional calcium channel.