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On the origin of a sustained increase in cytosolic Ca 2+ concentration after a toxic glutamate treatment of the nerve cell culture
Author(s) -
Khodorov B.,
Pinelis V.,
Golovina V.,
Fajuk D.,
Andreeva N.,
Uvarova T.,
Khaspekov L.,
Victorov I.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80132-e
Subject(s) - glutamate receptor , chemistry , biophysics , cytosol , calcium , endocrinology , medicine , biochemistry , biology , receptor , organic chemistry , enzyme
A sustained increase of cytosolic Ca 2+ concentration, [Ca 2+ ] i , (Ca 2+ plateau) was induced by a 15‐min treatment with 50 μM glutamate of cultured cerebellar granule cells and hippocampal neurons in a Mg 2+ ‐free solution. Plateau proved to be insensitive to inhibition of Na + o /Ca 2+ i exchange caused by removal external Na + in the post‐glutamate period. A ~ 10 5 ‐fold reduction of [Ca 2+ ] o (from 1.5 mM to 20 nM) in the post‐glutamate period caused in most cells only a slow and small decrease in [Ca 2+ ] i , although the same low‐Ca 2+ trial before glutamate treatment caused in hippocampal cells very quick blockade of spontaneous [Ca 2+ ] i oscillation and a decrease in the basal [Ca 2+ ] i . The results suggest that the Ca 2+ plateau is due to a suppression of the Ca 2+ extrusion from the cell (in particular via Na + /Ca 2+ exchange) rather than from a persistent increase in Ca 2+ permeability of neuronal membrane.