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Nuclear protein transport is functionally conserved between yeast and higher eukaryotes
Author(s) -
Wagner Philipp,
Hall Michael N.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80121-a
Subject(s) - nuclear transport , importin , microbiology and biotechnology , xenopus , nuclear protein , saccharomyces cerevisiae , yeast , nuclear pore , biology , cytoplasm , cell nucleus , biochemistry , gene , transcription factor
The ability of a yeast nuclear protein to be transported into the nucleus of a higher eukaryotic cell was investigated. Mcm1, a transcriptional activator protein from Saccharomyces cerevisiae , was microinjected into the cytoplasm of Xenopus laevis frog oocytes. Mcm1 was imported into the oocyte nucleus indicating that the machinery for nuclear transport is conserved from yeast to higher eukaryotes. Furthermore, by comparing the nuclear import of free proteins and protein‐gold complexes, we found that protein‐gold complex formation appears to partially and specifically inactivate the nuclear transport activity of Mcm1 and that nucleoplasmin is an exceptionally good nuclear import substrate.