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Diethyldithiocarbamate inhibits induction of macrophage NO synthase
Author(s) -
Mülsch Alexander,
Schray-Utz Beate,
Mordvintcev Peter I.,
Hauschildt Sunna,
Busse Rudi
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80111-7
Subject(s) - nitric oxide synthase , stimulation , lipopolysaccharide , transcription (linguistics) , microbiology and biotechnology , chemistry , signal transduction , atp synthase , gene expression , messenger rna , enzyme , biology , biochemistry , gene , endocrinology , linguistics , philosophy
We investigated whether sodium diethyldithiocarbamate (DETC), an inhibitor of the nuclear transcription factor kappa B (NFkappaB), modulates induction of NO synthase (NOS) in murine bone marrow‐derived macrophages. A short exposure (between 1 and 16 h) of L929‐cell medium‐preconditioned macrophages to E. coli lipopolysaccharide (LPS) significantly increased the level of NOS mRNA, and elicited NO formation as detected by electron spin resonance spectroscopy and by the release of nitrite. DETC (0.1–1 mM) present during stimulation with LPS prevented the increase in NOS mRNA and the expression of NOS activity. These findings suggest that NFkappaB is involved in the signal transduction pathway linking stimulation of macrophages by LPS with transcription of, the gene encoding inducible NOS.