Premium
Prostaglandin A 2 and Δ 12 ‐prostaglandin J 2 induce apoptosis in L1210 cells
Author(s) -
Kim In-Kyung,
Lee Jeong-Hwa,
Sohn Hae-Won,
Kim Ho-Shik,
Kim Sang-Ho
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80110-g
Subject(s) - prostaglandin , apoptosis , chemistry , prostaglandin d2 , l1210 cells , prostaglandin f , prostaglandin e , microbiology and biotechnology , biochemistry , biology , in vitro , cytotoxicity
Treatment of L1210 cells with prostaglandin A 2 (PGA 2 ) or 9‐deoxy‐Δ 9,12 ‐13,14‐dihydro PGD 2 (Δ 12 ‐PGJ 2 ) resulted in significant G2/M arrest and subsequent DNA fragmentation at concentrations that are cytotoxic to the cells. On agarose gel electrophoresis, DNA ladder formation was evident 24 h after the addition of Δ 12 ‐PGJ 2 and remained apparent through 72 h, whereas G2/M accumulation was observed 6 h after the treatment. When the morphology of cells was examined by electron microscopy, L1210 cells incubated with a cytotoxic dose of PGA 2 or Δ 12 ‐PGJ 2 for 24 h showed the characteristic morphological features of apoptosis such as chromatin condensation, nuclear fragmentation and formation of apoptotic body. Cycloheximide blocked the DNA fragmentation and morphological changes induced by Δ 12 ‐PGJ 2 . Our results suggest that these cyclopentenone PGs caused apoptotic cell death of L1210 cells which is preceded by G2/M accumulation and requires de novo protein synthesis.