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Cloning of a novel putative G‐protein‐coupled receptor (NLR) which is expressed in neuronal and lymphatic tissue
Author(s) -
Kouba Monika,
Vanetti Mirko,
Wang Xioamin,
Schäfer Martin,
Höllt Volker
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80102-z
Subject(s) - microbiology and biotechnology , lymphatic system , biology , northern blot , messenger rna , spleen , amino acid , cloning (programming) , receptor , blot , peptide sequence , homology (biology) , western blot , molecular cloning , gene , immunology , biochemistry , computer science , programming language
A novel G‐protein‐coupled receptor was isolated from mouse and rat neuronal and lymphatic tissues. The amino acid sequence of the rat receptor (rNLR) shows an overall homology of 80% to a recently cloned receptor from Burkitt's lymphoma cells (BLR1) which is exclusively expressed in lymphatic tissues [(1992) Eur. J. Immunol. 22, 2795]. Much less homology between rNLR and BLR1 was observed at the N‐terminus (about 40%), whereas rNLR and the mouse homologue mNLR show 92% amino acid identity. Northern blot analysis of NLR revealed a predominant 5.5 kb mRNA species in various brain regions and neuronal cell lines, whereas in the spleen a 3 kb transcript is predominant. This distribution suggests a role of NLR in the nervous and immune systems.